Ghana's Marburg outbreak was the second in West Africa. The first ever case of the virus was detected last year in Guinea, with no further cases identified.
"The Ghana Health Service (GHS) has officially declared Ghana free from the Marburg virus disease outbreak that was confirmed nearly two months ago," the presidency tweeted on Monday.
Ghana's government confirmed its first outbreak of the disease on July 17.
Three cases have been confirmed in total, of which two were fatal. The third was asymptomatic and recovered.
There have been a dozen major Marburg outbreaks since 1967, mostly in southern and eastern Africa. Fatality rates have varied from 24% to 88% in past outbreaks depending on the virus strain and case management, according to the World Health Organization.